A wide range of PCR assays, including conventional and real-time PCRs, are currently available for the detection of either Marteilia refringens or Bonamia sp. parasites, with heterogeneous level of validation. In order to facilitate the diagnosis of these two listed diseases, the European Union Reference Laboratory (EURL) for mollusc diseases has developed and validated a new multiplex TaqMan® PCR assay allowing the detection of both parasites in one step. The aim of this study is to assess the performances of this new assay, and to compare them with other currently recommended PCR assays.
An Inter Laboratory Comparison test was organized in 2017 (2017-ILC-01) to test the competency of participants regarding the detection of Bonamia sp. and Marteilia refringens in flat oyster (Ostrea edulis) tissues by PCR. Optionally, participants were invited to test the new multiplex PCR assay for the detection of Bonamia sp. and Marteilia refringens.
18 laboratories participated in the 2017-ILC-01 test and 17 laboratories agreed to test the new multiplex PCR assay .
The report of this study is available on the NRLS' Section / Proficiency test / Results and reports (limited access by login and password).