Standard Operating Procedures

Documents

Several National Reference Laboratories and Pathology Laboratories are currently building their Quality Management System based on ISO 17025 Standard ( General requirements for the competence of testing and calibration laboratories). Some of them are already accredited but most of them are seeking for accreditation by their respective accreditation bodies. Some of these laboratories, and more particularly those in the last countries joining the European Union, are asking for standardised procedures in histopathology.

Several pathologists having already some experience in the field of Quality Management are presently joining their efforts to produce Standard Operating Procedures. These SOPs can be used as templates by European laboratories and can help them when writing down their own documentation for Quality Management. These documents are available below in PDF files.

Histopathology

Diagnosis by histo-cytopathology of Bonamia spp. in flat oysters Ostrea spp. (5th edition, 2013)	Bonamia sp. v5
Diagnosis by histo-cytopathology of Marteilia spp.  in the flat oyster Ostrea edulis and the mussels Mytilus edulis and M. galloprovincialis (2nd edition, 2009)	Marteilia sp. 2
Diagnosis by histopathology of Perkinsus sp. in molluscs (1st edition, 2009)	Perkinsus sp. 1
Diagnosis by histo-cytopathology of Mikrocytos sp. in oysters (2nd edition, 2010)	Mikrocytos sp. 2
Diagnosis by histopathology of Haplosporidium sp. in oysters (1st edition, 2009)	Haplosporidium sp. 1
Molluscs processing for diagnosis by histology (3rd edition, May 2011)	Histology Process 3
Koehler illumination system for the microscope (1st edition, 2009)	Microscope setting

Molecular biology

Bonamia spp detection by Polymerase Chain Reaction and species characterisation by Restriction Fragment Length Polymorphism (1st edition, October2008)	Bonamia PCR-RFLP
Bonamia sp.detection and characterization by in situ hybridization (ISH) (1st edition, May 2013)	Bonamia HIS v1
Marteilia refringens detection and characterisation by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism ( 3rd edition, december 2011)	Marteilia PCR-RFLP_3
Marteilia refringens detection and typing by Real time Polymerase Chain Reaction (1st edition, May 2016)	Real Time PCR assay for the detection and typing of Marteilia refringens
Marteilia refringens and Bonamia sp. detection by Real time Polymerase Chain Reaction (1st edition, April 2017)	Real time PCR Marteilia refringens and Bonamia sp. 1
Marteilia refringens detection and characterization by in situ hybridization (ISH) ( 3rd edition, September 2011)	Marteilia ISH-3
OsHV-1 detection and quantification by Real Time Polymerase Chain Reaction (1st edition, March 2011). For additionnal information about OsHV-1 détection by RT-PCR see: J-F. Pépin (2013). Short Technical Report for OsHV-1 detection and quantification by Real Time Polynerase Chain Reaction using OsHV-1 DNA polymerase sequence	OsHV-1 RTPCR_1
Vibrio aestuarianus detection by Real Time Polymerase Chain Reaction (2nd edition, October 2015)	Vibrio aestuarianus detection by RT PCR
Vibrio splendidus and V. aestuarianus detection by Real Time Polymerase Chain Reaction (1st edition, October 2013)	Vibrio splendidus and V. aestuarianus RT PCR

Other techniques

Quantification of Perkinsus sp. infection intensity using Ray’s Fluid Thioglycolate Medium (RFTM) Method (1st edition, February 2011)	Perkinsus RFTM 1
Isolation of predominant bacteria in marine molluscs (1st edition, July 2015)	Isolation of predominant bacteria in marine molluscs

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Quality management and Accreditation

Diagnosis of mollusc diseases by histology or by using molecular biology tools (PCR, HIS) are now performed under a quality assurance system since 2003, but present histology and molecular laboratories are also used for research and students training purpose. Decision was taken in 2001 to build a new section - close to the present one - harbouring 3 new laboratories for mollusc diseases diagnosis by using histology, molecular biology and bacteriology techniques.

Construction of the new building started in late February 2006 and was finished in September 2006. The building was inaugurated on the 9th of February 2007 by Mr D. Bussereau, Minister of Agriculture and Fisheries and Mr J.-Y. Perrot, CEO of Ifremer. The EURL for mollusc diseases has been accredited by the Cofrac (Comité français d'accréditation) in October 2009 for the diagnosis by histo-cytopathology of Bonamia sp., Marteilia sp., Perkinsus sp. and Mikrocytos sp. in molluscs (bivalves and gastropods). The accreditation has been renewed in June 2013.