Proficiency tests in histopathology

Organisation

The European Union Reference Laboratory regularly organises proficiency tests for the detection of the listed and/or exotic parasites by means of histology. The goal of these proficiency tests is to establish that the examination of a given sample lead to the same conclusions in any laboratory within the European National Reference Laboratories network. Testing is based on an itinerant collection of hematoxylin and eosin stained histological sections of molluscs. Each participant receives slides with the name of the host species and has one week to read them and gives their results to the EURL. Part of the test can be performed using scanned slides available through the mScope® software. In this case, participant have one month to read the digitized slides. Once all laboratories have performed the test, the EURL produce a coded report with identification of the content of the slides, and a summary of the results of all laboratories. The participants are informed of their own laboratory code number and are thus able to compare their performance with that of other laboratories.

European proficiency tests already organised in histopathology

Year

Host

Pathogens

1998

Ostrea edulis

Bonamia ostreae, Marteilia refringens

2001

Ostrea edulis

Bonamia ostreae, Marteilia refringens

Crassostrea gigas

Mikrocytos mackini,
Marteilioides chungmuensis

Crassostrea virginica

Haplosporidium nelsoni, H. costale, Perkinsus marinus

Ruditapes philippinarum

Perkinsus olseni

Saccostrea commercialis

Mikrocytos roughleyii

2002-2003

Crassostrea gigas

Mikrocytos mackini

Haplosporidium nelsoni

Ostrea edulis

Bonamia ostreae

Marteilia refringens

Mytilus edulis

Marteilia refringens

Ruditapes philippinarum

Perkinsus olseni

 2005

Ostrea edulis

Bonamia ostreae, Marteilia refringens

Crassostrea gigas

Mikrocytos mackini,
Marteilioides chungmuensis,
Haplosporidium nelsoni

2007

Ostrea edulis

Bonamia ostreae, Marteilia refringens

Crassostrea virginica

Haplosporidium nelsoni, H. costale, Perkinsus marinus

Crassostrea gigas

Mikrocytos mackini, Haplosoridium nelsoni

2009

Ostrea edulis

Bonamia sp., Marteilia sp.

Crassostrea gigas, C. virginica

Perkinsus sp., Mikrocytos sp., Haplosporidium sp.

2012

Ostrea edulis

Bonamia sp., Marteilia sp.

Crassostrea gigas, C. virginica

Perkinsus sp., Mikrocytos sp., Haplosporidium sp.

2014

Ostrea edulis

Bonamia sp., Marteilia sp.

Crassostrea gigas, C. virginica

Perkinsus sp., Mikrocytos sp.

Mytilus edulis, M. galloprovincialis

Marteilia sp.

Ruditapes philippinarum, R. decussatus

Perkinsus sp., Mikrocytos sp.

2016

Ostrea edulis

Bonamia sp., Marteilia sp.

Crassostrea gigas, C. virginica

Perkinsus sp., Mikrocytos sp., Marteilia sp.

Mytilus edulis, M. galloprovincialis

Marteilia sp.

Ruditapes philippinarum, R. decussatus

Perkinsus sp., Mikrocytos sp., Marteilia sp.

2018

Ostrea edulis

Bonamia sp., Marteilia sp.

Crassostrea spp.

Perkinsus sp., Mikrocytos sp.

Ruditapes spp.

Perkinsus sp., Mikrocytos sp., Marteilia sp.

Mytilus spp. (scanned slides)

Marteilia sp.

More information available in the NRL's section > Proficiency test page (access limited to the European NRLs by login and password).

2020 Histo-cytopathology proficency test

A Inter Laboratory Comparison (ILC) test is organised in 2020 in order to test the competency of participants regarding the detection by histo-cytopathology of the following pathogens : Bonamia sp., Marteilia sp., Perkinsus sp., Mikrocytos sp. and Haplosporidium sp.

The test includes:

- 10 scanned H&E stained histological sections of flat oysters Ostrea edulis

- 10 scanned HemacolorR stained imprints of flat oysters Ostrea edulis

- 20 scanned H&E stained histological sections of cupped oysters Crassostrea spp.

In the context of the Covid19 pandemic, all the slides were scanned and the whole test will be available online via the mScope software in order to avoid any transport problem.

Planning:

Announcement : 12th of August 2020

Deadline for registration: 18th of September 2020

Start date: 1st of October 2020

End date: 15th of December 2020