Proficiency tests in molecular biology

Year

Technique

Host

Pathogen

Samples sent

2008

PCR

Ostrea edulis

Bonamia ostreae

Tissues of flat oysters (30)

2010

PCR-RFLP

Ostrea edulis

Marteilia refringens

Tissues of flat oysters (12) and mussels (12)

2011

RT-PCR

Crassostrea gigas

Herpes virus OsHV-1

Tissues of Pacific oysters (24)

2013

PCR & RT-PCR

Ostrea edulis

Bonamia sp.

Tissues of flat oysters (24)

The 2013 ILC also aimed at comparing different PCR techniques for the detection of Bonamia sp. and the characterization of the species B. ostreae and B. exitiosa

2015

RT-PCR

Crassostrea gigas

Vibrio aestuarianus

Tissues of Pacific oysters (24)

2017

PCR & RT-PCR

Ostrea edulis

Marteilia refringens &

Bonamia sp.

Tissues of flat oysters (24)

The 2017 ILC also aimed at assessing a new multiplex real-time PCR recently developed by the EURL for mollusc diseases, allowing the simultaneous detection of Marteilia refringens and Bonamia sp.

2019

PCR & RT-PCR

Ostrea edulis

Bonamia ostreae and B. exitiosa

DNA of flat oysters (24)

The 2019 ILC also aimed at evaluating a new multiplex real-time PCR recently developed by the EURL for mollusc diseases, allowing the simultaneous detection of Bonamia ostreae and Bonamia exitiosa

2021

RT-PCR

Crassostrea gigas

Vibrio aestuarianus

DNA of Pacific oysters (24)

Registration for the 2021 ILC test is open until the 7th of May 2021. Samples are expected to be sent mid-may 2021, participants will have one month to perfrom the test and report their results.

Reports are available in the "NRL's section > Proficiency test > Result and reports" page (access limited to the European NRLs by login and password).